| Hardcopy Reference: |
HH-10-07-06 |
| Title: |
A BIOSENSOR TO DETECT PENICILLIN RESIDUES IN FOOD |
| Author(s): |
M. Delwiche, E. Cox, B. Goddeeris, C. Van Dorpe, J. De Baerdemaeker, E. Decuypere, W. Sansen |
| Publication Year: |
2000 |
| Reference: |
Transactions of the ASAE VOL. 43(1): 153-159
2000 American Society of Agricultural Engineers 0001-2351 / 00 / 4301-153 |
| Country: |
USA |
| Summary: |
|
| Abstract: |
Avoidance of antibiotic residues is an essential element in the production of safe meat and dairy products. Our objectives in this research were to develop an enzyme immunoassay for â-lactam penicillins, design an automated sensor to implement this assay, and evaluate the detection performance. An antibody capture with antigen competition assay was developed to run in polystyrene cuvets, with a test time of 13-15 min. A photometric sensor consisting of an optical head,
electronic/fluidic board, and control computer was then designed to implement this assay. Results from manual assay tests on ampicillin in phosphate-buffered saline showed a slight reduction in optical density at 100 ng/mL and a significant reduction of 21% for 200 ng/mL. Sensor calibration tests gave similar results, indicating that the detection chemistry was successfully automated. Higher affinity or avidity antibodies are needed to detect U.S. and European Community residue
limits. |
| Database: |
Meat Quality |
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